Absract Carbendazim (methyl-2-benzimidazole carbamate), a metabolite of benomyl, is one of the most widespread environmental contaminants of major concern to human and animal reproductive health. The present investigation was undertaken to study the possible protective effect of linseed oil on carbendazim induced testicular toxicity. Adult albino male rats of the Sprague Dawley strain were administered with carbendazim (100 mg/(kg /day orally) . The control animals received vehicle (corn oil) alone. Also, four other groups of rats were treated by vitamin E (Vit E) (200mg/kg /day orally), carbendazim + Vit E, linseed oil (1.5 g/kg/day, orally), or carbendazim + linseed oil. All the treatments were continued for 48 days. After the treatment period, animals were euthanized, blood samples were taken, prostate and seminal vesicles were removed and weighed. Testes were also quickly removed, encapsulated and utilized for the estimation of the following parameters: sperm count, motility, viability, and abnormalities. Malondialdehyde and reduced glutathione, superoxide dismutase, and glutathione peroxidase anti oxidant biomarkers activities were estimated in the testes homogenate. Activities of testicular acetylcholine esterase and lactate dehydrogenase enzymes as well as serum testosterone levels were also determined.